Original Article
Year : 2017 | Volume : 1 | Issue : 2 | Page : 41 - 46
Alkaline phosphatase and lactate dehydrogenase enzyme activity in gingival crevicular fluid during orthodontic tooth movements
N. Yannawar Vijay, R. M. Dharma1, M. R. Dinesh1, Kalpesh Sharma2, Sunny Gupta3, Garima Chitakara1, Abhishek Singh Nayyar4
Department of Orthodontics and Dentofacial Orthopaedics, Dr. H.S.R.S.M. Dental College and Hospital, Hingoli, 4 Department of Oral Medicine and Radiology, Saraswati-Dhanwantari Dental College and Hospital and Post-Graduate Research Institute, Parbhani, Maharashtra, 1 Department of Orthodontics and Dentofacial Orthopaedics, DAPMRV Dental College and Hospital, Bangalore, Karnataka, 2 Department of Orthodontics and Dentofacial Orthopaedics, Geetanjali Dental and Research Institute, Udaipur, Rajasthan, India, 3 Department of Orthodontics and Dentofacial Orthopaedics, Faculty of Dentistry, Melaka Manipal Medical College, Malaysia
Address for correspondence:
Dr. N. Yannawar Vijay, Department of Orthodontics and Dentofacial Orthopaedics, Dr. H.S.R.S.M. Dental College and Hospital, Hingoli, Maharashtra, India.
E‐mail: yannawarvijay@gmail.com
Abstract
Background:
Bone remodeling is a biologic process involving an acute inflammatory response in periodontal tissues during orthodontic tooth movement. The early response of periodontal tissues to mechanical stress involves metabolic changes that allow tooth movement.
Aim of the Study:
The aim of the present study was to evaluate the changes in alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) enzyme activity in gingival crevicular fluid (GCF) when forces are applied during initial alignment (P1) and retraction (P2).
Settings and Design:
The study was performed in a hospital‐based population with an experimental design.
Materials and Methods:
Seventeen participants (11–21 years) included in the study, all requiring 1st premolar extractions were undergoing fixed orthodontic treatment. Maxillary canine and mandibular 2nd molar were used as test tooth (TT) and control tooth (CT) in each patient, respectively. On 14th day after start of each phase, 2 μL of GCF was collected from mesial side of both TT and CT for ALP and LDH activity determination using spectrophotometer.
Statistical Analysis:
Statistical analysis was performed with SPSS (version 13, SPSS Inc., Chicago, USA) package. The significance of differences in both phases at test or, at control site for LDH and ALP enzyme activity was evaluated by performing student’s paired t‐test. The significance of differences in between test and control sites in each phase separately was evaluated by performing student’s unpaired t‐test.
Results:
There was a variation in enzyme activity during orthodontic tooth movement. At TT, there was statistically significant increase in ALP and LDH activity in P2 as compared to P1 with P = 0.005 and P ≤ 0.001, respectively. At CT, there was no statistically significant difference in ALP and LDH activity in P1 and P2 with P = 0.054 and P = 0.061, respectively.
Conclusion:
This study concluded that ALP and LDH activity could be successfully estimated in the GCF and ALP and LDH activity in GCF reflects the biologic activity in the periodontium during orthodontic tooth movements.
Keywords: Alkaline phosphatase, gingival crevicular fluid, lactate dehydrogenase, orthodontic tooth movement, spectrophotometer
Financial support and sponsorship
Nil.
Conflicts of interest
There are no conflicts of interest.
How to cite this article:
Vijay NY, Dharma RM, Dinesh MR, Sharma K, Gupta S, Chitakara G, et al. Alkaline phosphatase and lactate dehydrogenase enzyme activity in gingival crevicular fluid during orthodontic tooth movements. Int J Clinicopathol Correl 2017;1:41-6.
