Original Article

Year : 2017 | Volume : 1 | Issue : 1 | Page : 13 - 15

Impregnation and Embedding using Bees Wax and Paraffin Wax in Oral Tissue Samples: A Comparative Study

J Vini Mary Antony, Pratibha Ramani, N Anuja, Herald J Sherlin, Gheena S, Abilasha R, Gifrina Jeyaraj, Don KR, Archana S

Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha University, Chennai, Tamil Nadu, India

Address for correspondence:

Dr. J Vini Mary Antony,

E‐mail: jemeninoantony26@gmail.com

Abstract

Aim: The aim of this study is to compare paraffin wax that is used as routine embedding media and beeswax in impregnation and embedding of oral tissues.

Materials and Methods:

Ten biopsy specimens were impregnated and embedded in paraffin wax, ten biopsy specimens were impregnated and embedded in beeswax. After manual processing, all sections were stained with Hematoxylin and Eosin to compare the effect of beeswax and paraffin wax based on the features of the integrity of the section, uniformity of the stain, staining which includes nuclear details, cytoplasmic details, and background staining.

Results:

Beeswax showed well impregnation and embedding of the tissues as well as the preservation of the nuclear details, good cytoplasmic appearance, good tissue architecture and no bad effect on staining characteristics of the tissue. In addition, beeswax reduced the time needed for wax cooling.

Conclusion:

Beeswax can be used as an alternative to paraffin wax. Thus, we recommended using beeswax in our laboratories as they are widely available.

Keywords: Beeswax, embedding, impregnation, paraffin

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

How to cite this article:

Antony JV, Ramani P, Anuja N, Sherlin HJ, Gheena S, Abilasha R, et al. Impregnation and embedding using bees wax and paraffin wax in oral tissue samples: A comparative study. Int J Orofac Biol 2017;1:13-5.