Original Research
Key words: anaerobic organism, anti-microbial, cytotoxicity, dental plaque, Moringa oleifera Lam, periodontitis.
Year : 2022 | Volume : 13 | Issue : 4 | Page : 1-13
Assessing the cytotoxic effect and antimicrobial activity of Moringa oleifera aqueous and ethanolic extract against oral pathogens extracted from periodontal and orthodontic patients – an in vitro study
Shanmugapriya Ramamurthy1 , Sheeja Varghese2 , Umarevathi Gopalakrishnan1 , Mahesh Kumar3 , Mayma Nathasha4 , Jeyaram Palinivel4
1Sri Venkateswara Dental College & Hospital, The TamilNadu Dr MGR Medical University, Chennai, India, 600 130, Research Scholar, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai 6000077. 2Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai 6000077. 3Karpagavinayaga Institute of Dental Sciences, Chengalpattu (DT) 603 308, 4Consultant Orthodontist,
Address for Correspondence
Dr.Shanmugapriya Ramamurthy
Sri Venkateswara Dental College & Hospital, The TamilNadu Dr MGR Medical University, Chennai, India, 600130,
Email Id: drshanpriya@gmail.com
Abstract:
Background:
Periodontitis is the result of inflammation caused due to the activity of microorganisms. The prevalence of anaerobic organisms is more when it comes to periodontal pockets and orthodontic patients. Plants with phytochemicals that could exert antimicrobial effects could aid in host modulation for management of periodontitis caused by these bacteria in periodontal and orthodontic patients.
Aim:
To assess the antimicrobial effect of aqueous extract of Moringa oleifera Lam (MOL) and cytotoxic effect of aqueous and ethanol extracts of MOL.
Materials and methods:
Moringa oleifera Lam. extracts were prepared by maceration. Subgingival plaque samples were collected, and microorganisms were cultured in anaerobic environment. The microorganisms were treated with the extracts and minimum inhibitory concentration and minimum bactericidal concentration was assessed. The cytotoxic effects were assessed by brine shrimp assay.
Results:
Aqueous extract showed antimicrobial effect in dose and time dependent manner and both extracts exhibited cytotoxic effects in a dose and time dependent manner.
Summary and Conclusion:
The antimicrobial effect of MOL could be utilized to develop a nature derived local drug delivery system for treating plaque induced periodontitis in different clinical situations.
Key words: anaerobic organism, anti-microbial, cytotoxicity, dental plaque, Moringa oleifera Lam, periodontitis.
Conflict of interest:
No conflict of interest among authors in this study.
Sources of Funding:
Nil
How to Cite this Article: Assessing the cytotoxic effect and antimicrobial activity of Moringa Oleifera aqueous and ethanolic extract against oral pathogens extracted from periodontal and orthodontic patients – An In vitro study. Int J Orthod Rehabil.,13(4):1-13.