Original Article
Keywords:
Antibacterial, Biofilm, Extract, Leaf, Plant.
Year : 2023 | Volume : 14 | Issue : 3 | Page : 11-19
Evaluation of Antibacterial and Antibiofilm Activity of Cucurbita Maxima Leaf Extract Against Streptococcus Mutans Isolated from Orthodontic Patients
Ravindra Kumar Jain1 , Mathew Thomas Maliael2 , Pitchaipillai Sankar Ganesh3
1Professor, 2Post Graduate, Department of Orthodontics, 3Professor, Department of Microbiology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai – 77
Address for Correspondence:
Dr. Ravindra Kumar Jain
Professor and Head Department of Orthodontics and Dentofacial Orthopedics Saveetha Dental College and Hospital Saveetha Institute of Medical and Technical Sciences Chennai – 77.
Mail: ravindrakumar@saveetha.com
Abstract
Aim
The aim of this investigation was to evaluate the antimicrobial activity and anti-biofilm activity of Cucurbita Maxima leaf extract on Streptococcus mutans isolated from dental plaque of subjects undergoing orthodontic treatment.
Methodology
Dental plaque samples were collected from out patients undergoing orthodontic treatment. From the plaque samples Biochemical Characterisation of Streptococcus mutans then Collection of Medicinal Plants was done followed by Preparation of Solvent Extract and stock Solution. Evaluation of Minimum Inhibitory Concentration (MIC) of the plant extract and Biofilm Inhibition Assay with Gas Chromatography-Mass Spectrum Analysis (GCMS) was carried out.
Results
The MIC was identified to be 1.25mg/ml. The biofilm inhibition assay showed that the extract did not inhibit the formation of the biofilm. The GCMS analysis identified twenty-five constituent compounds from the crude extract.
Conclusion
The C. maxima leaf extract showed antibacterial activity against S. mutans and the minimum inhibitory concentration was identified at 1.25mg/ml. The extract did not inhibit the formation of biofilm.
Keywords: Antibacterial, Biofilm, Extract, Leaf, Plant.
CONFLICT OF INTEREST
The authors have no conflict of interest to declare.
FUNDING
No funding was obtained for this study
How to cite this article: Evaluation of Antibacterial and Antibiofilm Activity of Cucurbita Maxima Leaf Extract Against Streptococcus Mutans Isolated from Orthodontic Patients. Int J Orthod Rehabil 2023; 14 (3) 11 -19. Doi: 10.56501/intjorthodrehabil.v14i3.763.