Original Research

Year : 2023 | Volume : 6 | Issue : 2| Page : 23-30

COMPARISON OF ROUTINE XYLENE AND DISH WASHING SOLUTION WITH LEMON WATER USED AS DEPARAFFINIZING AGENTS

1Aiman Zafar

RoyaleKalindi hospital. 89 pocket-1 Jasola Vihar, New Delhi-110025

Address for Correspondence:

Dr. Aiman Zafar

Royale Kalindi Hospital,89 Pocket –1 Jasola Vihar, New Delhi –110025

Mail ID:aimanzafar946@gmail.com

Abstract

Background:

Xylene is an aromatic hydrocarbon known for its wide usage in tissue processing which has many hazardous effects.

Objectives:

To compare the efficacy of xylene free sections deparaffinized with dish washing soap and diluted lemon water and compare them.

Materials and Methods:

Using twenty paraffin embedded tissue blocks, three sections each were prepared. One section was stained with conventional H &E method (Group A) and the other two sections with xylene-free (XF) H & E (Groups B and C). Staining characteristics were compared with xylene and scoring was given.

Result:

There is appreciable nuclear stain, cytoplasmic staining, staining clarity with dishwash and diluted lemon water solution with p value <0.001 in comparison with xylene.

Conclusion:

This study shows good result in terms of nuclear staining, cytoplasmic staining, and clarity of staining, liquid soap with lemon can be used as alternate to xylene.

Financial support and sponsorship:

Nil

Conflicts of interest:

There are no conflicts of interest

How to cite this article: Zafar, A. (2023). COMPARISON OF ROUTINE XYLENE AND DISH WASHING SOLUTION WITH LEMON WATER USED AS DEPARAFFINIZING AGENTS: Original research. International Journal of Head and Neck Pathology, 6(2), 23–30. https://doi.org/10.56501/intjheadneckpathol.v6i2.994